| First Author | Shelburne CP | Year | 2003 |
| Journal | Blood | Volume | 102 |
| Issue | 4 | Pages | 1290-7 |
| PubMed ID | 12714518 | Mgi Jnum | J:84889 |
| Mgi Id | MGI:2670544 | Doi | 10.1182/blood-2002-11-3490 |
| Citation | Shelburne CP, et al. (2003) Stat5 expression is critical for mast cell development and survival. Blood 102(4):1290-7 |
| abstractText | Interleukin-3 (IL-3) and stem cell factor (SCF) are important mast cell growth and differentiation factors. Since both cytokines activate the transcription factor signal transducer and activator of transcription 5 (Stat5), a known regulator of proliferation and survival, we investigated the effects of Stat5 deficiency on mast cell development and survival. Bone marrow-derived mast cell (BMMC) populations cultured from Stat5A/B-deficient mice survived in IL-3 + SCF, but not in either cytokine alone. These cells demonstrated reduced expression of Bcl-2, Bcl-x(L), cyclin A2, and cyclin B1, with increased apoptosis and delayed cell cycle progression during IL-3 or SCF culture. Finally, the absence of Stat5 resulted in loss of in vivo mast cell development, as judged by assessments of Stat5-deficient mice and transplantation of Stat5-deficient bone marrow cells to mast cell-deficient recipient mice. These results indicate that Stat5A and Stat5B are critical regulators of in vitro and in vivo mast cell development and survival. |
