| First Author | Heape AM | Year | 1996 |
| Journal | Neurochem Int | Volume | 29 |
| Issue | 6 | Pages | 607-22 |
| PubMed ID | 9113128 | Mgi Jnum | J:40686 |
| Mgi Id | MGI:708064 | Doi | 10.1016/s0197-0186(96)00059-9 |
| Citation | Heape AM, et al. (1996) Pathways of incorporation of fatty acid into glycerolipids of the murine peripheral nervous system in vivo: alterations in the dysmyelinating mutant trembler mouse. Neurochem Int 29(6):607-22 |
| abstractText | In vivo glycerolipid metabolism was studied in sciatic nerves of normal and Trembler mice. The results showed that two kinetically independent pathways were implicated in the labeling of diacylglycerophospholipids from [3H]palmitate: the Kennedy pathway and a 'direct acylation' pathway. In normal nerves, 45% of the glycerophospholipids were labeled, with a rate constant k3 = 3.9 x 10(-3) min-1, from phosphatidic acid and diacylglycerol intermediates, themselves formed with a rate constant of k1 = 0.24 min-1 from a free 3H-fatty acid pool, FFA1, that represents 45% of the total injected label. The remaining 55% of the glycerophospholipids were labeled from a kinetically distinct free 3H-fatty acid pool, FFA2, with a rate constant of k4 = 9.8 x 10(-2) min-1, via a process that does not implicate a detectably labeled metabolic intermediate ('direct acylation'). Glycerophospholipid labeling via the Kennedy pathway in the Trembler mouse sciatic nerves was reduced to 75% of the normal level, while labeling via the 'direct acylation' pathway was increased 1.4-fold. The values of the rate constants for free 3H-fatty acid utilisation (k1 and k4) were both increased about 2.5-fold, while that of glycerophospholipid formation from diacylglycerol (k3) was close to normal. |
