| First Author | McKimpson WM | Year | 2022 |
| Journal | STAR Protoc | Volume | 3 |
| Issue | 2 | Pages | 101287 |
| PubMed ID | 35719268 | Mgi Jnum | J:350251 |
| Mgi Id | MGI:7662424 | Doi | 10.1016/j.xpro.2022.101287 |
| Citation | McKimpson WM, et al. (2022) Inducing and measuring apoptotic cell death in mouse pancreatic beta-cells and in isolated islets. STAR Protoc 3(2):101287 |
| abstractText | Type 2 diabetes is mediated by insulin resistance and pancreatic beta-cell failure, the latter reflecting a combination of beta-cell dysfunction, dedifferentiation, and apoptosis. Quantification of beta-cell apoptosis in diabetes can be challenging both with respect to methodology and selection of clinically relevant inducers and readouts. This protocol describes approaches to measure cell death in immortalized beta-cells, primary mouse islet preparations, and pancreatic tissue. The resulting information may be useful for mechanistic studies and assessment of the contribution of beta-cell death to pathogenesis. For complete details on the use and execution of this protocol, please refer to McKimpson et al. (2021). |
