| First Author | Gould KA | Year | 1996 |
| Journal | Genetics | Volume | 144 |
| Issue | 4 | Pages | 1777-85 |
| PubMed ID | 8978063 | Mgi Jnum | J:37223 |
| Mgi Id | MGI:84625 | Doi | 10.1093/genetics/144.4.1777 |
| Citation | Gould KA, et al. (1996) Genetic evaluation of candidate genes for the Mom1 modifier of intestinal neoplasia in mice. Genetics 144(4):1777-85 |
| abstractText | As genetic mapping of quantitative trait loci (QTL) becomes routine, the challenge is to identify the underlying genes. This paper develops rigorous genetic tests for evaluation of candidate genes for a QTL, involving determination of allelic status in inbred strains and fine-structure genetic mapping. For the Mom1 modifier of intestinal adenomas caused by ApcMin, these tests are used to evaluate two candidate genes: Pla2g2a, a secretory phospholipase, and Rap1GAP, a GTPase activating protein. Rap1GAP passes the first test but is excluded by a single fine-structure recombinant. Pla2g2a passes both tests and is a strong candidate for Mom1. Significantly, we also find that ApcMin-induced adenomas remain heterozygous for the Mom1 region, consistent with Mom1 acting outside the tumor lineage and encoding a secreted product. |
