| First Author | Jala VR | Year | 2021 |
| Journal | Int J Cancer | PubMed ID | 33497467 |
| Mgi Jnum | J:303955 | Mgi Id | MGI:6514568 |
| Doi | 10.1002/ijc.33477 | Citation | Jala VR, et al. (2021) Absence of CCR2 reduces spontaneous intestinal tumorigenesis in the Apc(Min) (/+) mouse model. Int J Cancer |
| abstractText | The biological activities of chemokine (C-C motif) ligand 2 (CCL2) are mediated via C-C chemokine receptor-2 (CCR2). Increased CCL2 level is associated with metastasis of many cancers. In our study, we investigated the role of the CCL2/CCR2 axis in the development of spontaneous intestinal tumorigenesis using the Apc(Min/+) mouse model. Ablation of CCR2 in Apc(Min/+) mice significantly increased the overall survival and reduced intestinal tumor burden. Immune cell analysis showed that CCR2(-/-) Apc(Min/+) mice exhibited significant reduction in the myeloid cell population and increased interferon gamma (IFN-gamma) producing T cells both in spleen and mesenteric lymph nodes compared to Apc(Min/+) mice. The CCR2(-/-) Apc(Min/+) tumors showed significantly reduced levels of interleukin (IL)-17 and IL-23 and increased IFN-gamma and Granzyme B compared to Apc(Min/+) tumors. Transfer of CCR2(+/+) Apc(Min/+) CD4(+) T cells into Rag2(-/-) mice led to development of colitis phenotype with increased CD4(+) T cells hyper proliferation and IL-17 production. In contrast, adoptive transfer of CCR2(-/-) Apc(Min/+) CD4(+) T cells into Rag2(-/-) mice failed to enhance colonic inflammation or IL-17 production. These results a suggest novel additional role for CCR2, where it regulates migration of IL-17 producing cells mediating tumor-promoting inflammation in addition to its role in migration of tumor associated macrophages. |
