| First Author | Jha A | Year | 2015 |
| Journal | Sci Signal | Volume | 8 |
| Issue | 399 | Pages | ra103 |
| PubMed ID | 26486172 | Mgi Jnum | J:259046 |
| Mgi Id | MGI:6140583 | Doi | 10.1126/scisignal.aac7538 |
| Citation | Jha A, et al. (2015) Essential roles for Cavbeta2 and Cav1 channels in thymocyte development and T cell homeostasis. Sci Signal 8(399):ra103 |
| abstractText | Calcium ions (Ca(2+)) are important in numerous signal transduction processes, including the development and differentiation of T cells in the thymus. We report that thymocytes have multiple types of pore-forming alpha subunits and regulatory beta subunits that constitute voltage-gated Ca(2+) (Cav) channels. In mice, T cell-specific deletion of the gene encoding the beta2 regulatory subunit of Cav channels (Cacnb2) reduced the abundances of the channels Cav1.2 and Cav1.3 (both of which contain pore-forming alpha1 subunits) and impaired T cell development, which led to a substantial decrease in the numbers of thymocytes and peripheral T cells. Similar to the effect of Cacnb2 deficiency, pharmacological blockade of pore-forming Cav1alpha subunits reduced the sustained Ca(2+) influx in thymocytes upon stimulation of the T cell receptor, decreased the abundance of the transcription factor NFATc3, inhibited the proliferation of thymocytes in vitro, and led to lymphopenia in mice. Together, our data suggest that Cav1 channels are conduits for the sustained Ca(2+) influx that is required for the development of T cells. |
