| First Author | Johnson EE | Year | 2024 |
| Journal | Hum Mol Genet | Volume | 33 |
| Issue | 7 | Pages | 594-611 |
| PubMed ID | 38181046 | Mgi Jnum | J:350278 |
| Mgi Id | MGI:7662672 | Doi | 10.1093/hmg/ddad206 |
| Citation | Johnson EE, et al. (2024) Retention of stress susceptibility in the mdx mouse model of Duchenne muscular dystrophy after PGC-1alpha overexpression or ablation of IDO1 or CD38. Hum Mol Genet 33(7):594-611 |
| abstractText | Duchenne muscular dystrophy (DMD) is a lethal degenerative muscle wasting disease caused by the loss of the structural protein dystrophin with secondary pathological manifestations including metabolic dysfunction, mood and behavioral disorders. In the mildly affected mdx mouse model of DMD, brief scruff stress causes inactivity, while more severe subordination stress results in lethality. Here, we investigated the kynurenine pathway of tryptophan degradation and the nicotinamide adenine dinucleotide (NAD+) metabolic pathway in mdx mice and their involvement as possible mediators of mdx stress-related pathology. We identified downregulation of the kynurenic acid shunt, a neuroprotective branch of the kynurenine pathway, in mdx skeletal muscle associated with attenuated peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1alpha) transcriptional regulatory activity. Restoring the kynurenic acid shunt by skeletal muscle-specific PGC-1alpha overexpression in mdx mice did not prevent scruff -induced inactivity, nor did abrogating extrahepatic kynurenine pathway activity by genetic deletion of the pathway rate-limiting enzyme, indoleamine oxygenase 1. We further show that reduced NAD+ production in mdx skeletal muscle after subordination stress exposure corresponded with elevated levels of NAD+ catabolites produced by ectoenzyme cluster of differentiation 38 (CD38) that have been implicated in lethal mdx response to pharmacological beta-adrenergic receptor agonism. However, genetic CD38 ablation did not prevent mdx scruff-induced inactivity. Our data do not support a direct contribution by the kynurenine pathway or CD38 metabolic dysfunction to the exaggerated stress response of mdx mice. |
