| First Author | Zhai L | Year | 2017 |
| Journal | Cell Death Dis | Volume | 8 |
| Issue | 3 | Pages | e2707 |
| PubMed ID | 28358363 | Mgi Jnum | J:328174 |
| Mgi Id | MGI:6844449 | Doi | 10.1038/cddis.2017.128 |
| Citation | Zhai L, et al. (2017) miR-127 enhances myogenic cell differentiation by targeting S1PR3. Cell Death Dis 8(3):e2707 |
| abstractText | MicroRNAs (miRNAs) have recently been implicated in muscle stem cell function. miR-127 is known to be predominantly expressed in skeletal muscle, but its roles in myogenic differentiation and muscle regeneration are unknown. Here, we show that miR-127 is upregulated during C2C12 and satellite cell (SC) differentiation and, by establishing C2C12 cells stably expressing miR-127, demonstrate that overexpression of miR-127 in C2C12 cells enhances myogenic cell differentiation. To investigate the function of miR-127 during muscle development and regeneration in vivo, we generated miR-127 transgenic mice. These mice exhibited remarkably accelerated muscle regeneration compared with wild-type mice by promoting SC differentiation. Mechanistically, we demonstrated that the gene encoding sphingosine-1-phosphate receptor 3 (S1PR3), a G-protein-coupled receptor for sphingosine-1-phosphate, is a target of miR-127 required for its function in promoting myogenic cell differentiation. Importantly, overexpression of miR-127 in muscular dystrophy model mdx mice considerably ameliorated the disease phenotype. Thus, our findings suggest that miR-127 may serve as a potential therapeutic target for the treatment of skeletal muscle disease in humans. |
