| First Author | Lagasse E | Year | 1997 |
| Journal | Cell | Volume | 89 |
| Issue | 7 | Pages | 1021-31 |
| PubMed ID | 9215625 | Mgi Jnum | J:41240 |
| Mgi Id | MGI:893319 | Doi | 10.1016/s0092-8674(00)80290-1 |
| Citation | Lagasse E, et al. (1997) Enforced expression of Bcl-2 in monocytes rescues macrophages and partially reverses osteopetrosis in op/op mice. Cell 89(7):1021-31 |
| abstractText | Osteopetrotic (op/op) mice lack functional M-CSF and have depressed levels of macrophages and osteoclasts. We prepared transgenic mice (hMRP8bcl-2) that express human Bcl-2 in monocytes. In vitro hMRP8bcl-2 monocytes do not undergo apoptosis in the absence of serum and M-CSF, while op/op and wild-type monocytes die. These Bcl-2-expressing monocytes spontaneously undergo macrophage differentiation. In vivo, the op/op hMRP8bcl-2 mice show significant replenishment of tissue macrophages. Their long bone osteopetrosis is largely reversed, and extensive medullary hematopoiesis appears in the bone marrow. We propose that M-CSF augments monocyte survival, permitting them to respond to internal and external cues for their differentiation. |
