| First Author | Kishimoto H | Year | 1998 |
| Journal | J Exp Med | Volume | 187 |
| Issue | 9 | Pages | 1427-38 |
| PubMed ID | 9565635 | Mgi Jnum | J:112083 |
| Mgi Id | MGI:3655458 | Doi | 10.1084/jem.187.9.1427 |
| Citation | Kishimoto H, et al. (1998) A role for Fas in negative selection of thymocytes in vivo. J Exp Med 187(9):1427-38 |
| abstractText | To seek information on the role of Fas in negative selection, we examined subsets of thymocytes from normal neonatal mice versus Fas-deficient lpr/lpr mice injected with graded doses of antigen. In normal mice, injection of 1-100 microg of staphylococcal enterotoxin B (SEB) induced clonal elimination of SEB-reactive Vbeta8+ cells at the level of the semi-mature population of HSAhi CD4+ 8- cells found in the thymic medulla; deletion of CD4+ 8+ cells was minimal. SEB injection also caused marked elimination of Vbeta8+ HSAhi CD4+ 8- thymocytes in lpr/lpr mice. Paradoxically, however, elimination of these cells in lpr/lpr mice was induced by low-to-moderate doses of SEB (</=1 microg) but not by high doses (100 microg). Similar findings applied when T cell receptor transgenic mice were injected with specific peptide. These findings suggest that clonal elimination of semi-mature medullary T cells is Fas independent at low doses of antigen but Fas dependent at high doses. Previous reports documenting that negative selection is not obviously impaired in lpr/lpr mice could thus reflect that the antigens studied were expressed at only a low level. |
