| First Author | Gupta M | Year | 2005 |
| Journal | J Immunol | Volume | 174 |
| Issue | 7 | Pages | 4198-202 |
| PubMed ID | 15778381 | Mgi Jnum | J:97965 |
| Mgi Id | MGI:3576826 | Doi | 10.4049/jimmunol.174.7.4198 |
| Citation | Gupta M, et al. (2005) CD8-mediated protection against Ebola virus infection is perforin dependent. J Immunol 174(7):4198-202 |
| abstractText | CD8 T cells have been shown to play an important role in the clearance and protection against fatal Ebola virus infection. In this study, we examined the mechanisms by which CD8 T cells mediate this protection. Our data demonstrate that all normal mice infected s.c. with a mouse-adapted Ebola virus survived the infection, as did 100% of mice deficient in Fas and 90% of those deficient in IFN-gamma. In contrast, perforin-deficient mice uniformly died after s.c. challenge. Perforin-deficient mice failed to clear viral infection even though they developed normal levels of neutralizing anti-Ebola Abs and 5- to 10-fold higher levels of IFN-gamma than control mice. Using MHC class I tetramers, we have also shown that perforin-deficient mice have 2- to 4-fold higher numbers of Ebola-specific CD8s than control mice. These findings suggest that the clearance of Ebola virus is perforin-dependent and provide an additional example showing that this basic immunologic mechanism is not limited to the clearance of noncytopathic viruses. |
