| First Author | Mori K | Year | 1994 |
| Journal | Autoimmunity | Volume | 17 |
| Issue | 1 | Pages | 49-57 |
| PubMed ID | 7517710 | Mgi Jnum | J:19862 |
| Mgi Id | MGI:67985 | Doi | 10.3109/08916939409014658 |
| Citation | Mori K, et al. (1994) In vivo cytokine gene expression in various T cell subsets of the autoimmune MRL/Mp-lpr/lpr mouse. Autoimmunity 17(1):49-57 |
| abstractText | We have used the reverse transcriptase polymerase chain reaction (RT-PCR) technique to assess the expression of cytokine genes in various T cell subsets of autoimmune-prone mice. Our study confirmed the previously described features in lpr mice that IFN-gamma, TNF-alpha, and TNF-beta were transcribed by various T cell subsets. We in this study demonstrated that double negative (DN) T cells, the major cell population in lpr mice, failed to express interleukin-3 (IL-3), IL-4, IL-5, and IL-6 genes that influence B cell growth and activation. In contrast, DN T cells expressed Eta-1 gene that is shown to augment polyclonal activation of B cells and immunoglobulin production. Thus, it is conceivable that T cell-derived B cell-stimulatory activities in MRL/lpr mice can be attributed to Eta-1, rather than IL-3, IL-4, IL-5 or IL-6. We also demonstrated that CD4+ T cells infiltrating into kidney tissues of MRL/lpr mice expressed various cytokine genes such as IL-1, IL-5, IL-6, IFN-gamma, TNF-alpha, TNF-beta and TGF-beta. |
