| First Author | Hayashi Y | Year | 1995 |
| Journal | Arthritis Rheum | Volume | 38 |
| Issue | 8 | Pages | 1077-84 |
| PubMed ID | 7639803 | Mgi Jnum | J:28856 |
| Mgi Id | MGI:76388 | Doi | 10.1002/art.1780380809 |
| Citation | Hayashi Y, et al. (1995) Biased T cell receptor V beta gene usage during specific stages of the development of autoimmune sialadenitis in the MRL/lpr mouse model of Sjogren's syndrome. Arthritis Rheum 38(8):1077-84 |
| abstractText | OBJECTIVE. To analyze the repertoire of T cell receptor (TCR) V beta gene transcribed and expressed within the autoimmune lesions of the salivary gland in the MRL/lpr mouse model of Sjogren's syndrome. METHODS. Monoclonal antibodies (MAb) were used to determine the prevalence of selected V gene elements on T cell infiltrates from salivary glands of MRL/lpr mice. To analyze TCR V beta gene usage, we used reverse-transcriptase polymerase chain reaction (RT-PCR) and single-strand conformational polymorphism (SSCP) analyses. RESULTS. A predominance of V beta 8+ T cells was detected within the inflammatory lesions during development of autoimmune disease (confirmed by flow cytometry). RT-PCR analysis revealed that in autoimmune sialadenitis, the predominant expression of the V beta 8 gene segment began in the early stages of disease (2-month-old mice) and increased over time. Extensive age-related diversity of TCR V beta gene usage was also observed. SSCP analysis demonstrated a distinct and common binding pattern of the V beta 8 gene PCR product from the cell infiltrates during the course of the disease. CONCLUSION. Our data suggest that in the MRL/lpr mouse model of Sjogren's syndrome, there is restricted usage of TCR V beta elements according to the stage of the disease, and that V beta 8 are probably used preferentially in the recognition of a single unknown self antigen in the salivary gland. |
