| First Author | Rao A | Year | 2007 |
| Journal | J Neurosci | Volume | 27 |
| Issue | 51 | Pages | 14199-204 |
| PubMed ID | 18094259 | Mgi Jnum | J:129267 |
| Mgi Id | MGI:3768959 | Doi | 10.1523/JNEUROSCI.4934-07.2007 |
| Citation | Rao A, et al. (2007) Gbeta5 is required for normal light responses and morphology of retinal ON-bipolar cells. J Neurosci 27(51):14199-204 |
| abstractText | Gbeta5 exists as two splice variants, Gbeta5-S and Gbeta5-L, which interact with and stabilize the R7 members of the regulators of G-protein signaling (RGSs): RGS6, RGS7, RGS9, and RGS11. Although the role of Gbeta5-L and RGS9-1 is established in photoreceptors, the physiological functions of Gbeta5-S and other R7 RGS proteins remain unclear. We found that the electroretinogram of Gbeta5-/- mice lacks the b-wave component and that Gbeta5-S and RGS11 colocalize with Go alpha at the tips of the ON-bipolar cell dendrites. Unexpectedly, we found a significant reduction in the number of synaptic triads in the outer plexiform layer (OPL) of the Gbeta5-/- mice, which is evident at postnatal day 14. Transgenic expression of Gbeta5-L in rods failed to rescue the b-wave or the OPL defects. These results indicate that Gbeta5-S is indispensable for OPL integrity and normal light responses of the retina. |
