| First Author | Rothman TP | Year | 1996 |
| Journal | Dev Biol | Volume | 178 |
| Issue | 2 | Pages | 498-513 |
| PubMed ID | 8812145 | Mgi Jnum | J:35512 |
| Mgi Id | MGI:82959 | Doi | 10.1006/dbio.1996.0234 |
| Citation | Rothman TP, et al. (1996) Increased expression of laminin-1 and collagen (IV) subunits in the aganglionic bowel of ls/ls, but not c-ret -/- mice. Dev Biol 178(2):498-513 |
| abstractText | Extracellular matrix molecules, including laminin, affect the development of enteric neurons and accumulate in the aganglionic colon of ls/ls mice, Quantitative Northern analysis revealed that mRNAs encoding the beta 1 and gamma 1 subunits of laminin and collagens alpha 1(IV) and alpha 2(IV) are increased in the colons of ls/ls mice. Transcripts of laminin alpha 1 were evaluated quantitatively with reverse transcription and the competitive polymerase chain reaction (RT-cPCR). The abundance of Laminin alpha 1 transcripts tvas developmentally regulated, but greater in the ls/ls than the wild-type colon at each age examined, In situ hybridization revealed that transcripts in the colon encoding laminin alpha 1 and beta and collagen alpha 2(IV) were initially expressed in the endoderm, but by E15, expression shifted to cells of the colonic mesenchyme (ls/ ls >wild type) where crest-derived cells migrate. The expression of laminin alpha 1 was examined in the totally aganglionic intestine of E15 and newborn c-ret-/-mice, to determine whether an increase occurs when neurogenesis fails independently of the ls/ls defect. RT-cPCR revealed no difference from control in mRNA encoding laminin alpha 1 in the c-ret-/-colon in either E15 or newborn animals, The accumulation of immunohistochemically demonstrable laminin that is prominent in the newborn ls/ls colon could not be detected in that of c-ret-/-animals. These observations suggest that transcripts encoding laminin-1 and collagen (IV) are increased in the colon and surrounding pelvic mesenchyme of ls/ls mice because of an intrinsic lesion, rather than a secondary consequence of aganglionosis. The data are compatible with the hypothesis that the increased expression of laminin-1 contributes to the failure of crest-derived cells to complete their colonization of the ls/ls colon. (C) 1996 Academic Press, Inc. |
