| First Author | O'Shaughnessy PJ | Year | 1994 |
| Journal | Mol Cell Endocrinol | Volume | 104 |
| Issue | 2 | Pages | 133-8 |
| PubMed ID | 7527351 | Mgi Jnum | J:19961 |
| Mgi Id | MGI:68080 | Doi | 10.1016/0303-7207(94)90115-5 |
| Citation | O'Shaughnessy PJ, et al. (1994) Development of cytochrome P-450 side chain cleavage mRNA levels in neonatal ovaries of normal and hypogonadal (hpg) mice. Mol Cell Endocrinol 104(2):133-8 |
| abstractText | Cytochrome P-450 side chain cleavage (P-450scc) is vital for biosynthesis of gonadal steroids. In this study changes in P-450scc mRNA levels and cholesterol side-chain cleavage (CSCC) enzyme activity have been measured during development in the ovary of the normal mouse and the hypogonadal (hpg) mouse which lacks circulating gonadotrophins. Levels of P-450scc mRNA were measured using a semi-quantitative reverse transcription-polymerase chain reaction technique for which part of the 5' sequence of a mouse P-450scc cDNA was sequenced. On the day of birth P-450scc mRNA was present at low levels in the mouse ovary. Thereafter there was no change in P-450scc mRNA levels for 5 days after which time levels increased significantly to reach a peak around day 10. Activity of CSCC showed a similar pattern of development although activity was not detectable on days 1 and 3. In the hpg mouse P-450scc mRNA levels were normal on day 1 but did not increase thereafter up to 15 days. These results show that there is gonadotrophin-independent expression of P-450scc mRNA in the mouse ovary at birth when only primordial follicles and stromal tissue are present. As the ovary develops after 5 days changes in P-450scc mRNA levels become gonadotrophin-dependent and coincide with maturation of secondary follicles. |
