| First Author | Yoshinaga S | Year | 2021 |
| Journal | iScience | Volume | 24 |
| Issue | 4 | Pages | 102277 |
| PubMed ID | 33851097 | Mgi Jnum | J:325526 |
| Mgi Id | MGI:6717505 | Doi | 10.1016/j.isci.2021.102277 |
| Citation | Yoshinaga S, et al. (2021) Comprehensive characterization of migration profiles of murine cerebral cortical neurons during development using FlashTag labeling. iScience 24(4):102277 |
| abstractText | In the mammalian cerebral neocortex, different regions have different cytoarchitecture, neuronal birthdates, and functions. In most regions, neuronal migratory profiles are speculated similar based on observations using thymidine analogs. Few reports have investigated regional migratory differences from mitosis at the ventricular surface. In this study, we applied FlashTag technology, in which dyes are injected intraventricularly, to describe migratory profiles. We revealed a mediolateral regional difference in the migratory profiles of neurons that is dependent on developmental stage; for example, neurons labeled at embryonic day 12.5-15.5 reached their destination earlier dorsomedially than dorsolaterally, even where there were underlying ventricular surfaces, reflecting sojourning below the subplate. This difference was hardly recapitulated by thymidine analogs, which visualize neurogenic gradients, suggesting a biological significance different from the neurogenic gradient. These observations advance our understanding of cortical development and the power of FlashTag in studying migration and are thus resources for future neurodevelopmental studies. |
