| First Author | Watanabe M | Year | 1996 |
| Journal | Neurosci Res | Volume | 26 |
| Issue | 4 | Pages | 335-43 |
| PubMed ID | 9004271 | Mgi Jnum | J:38078 |
| Mgi Id | MGI:85467 | Doi | 10.1016/s0168-0102(96)01115-7 |
| Citation | Watanabe M, et al. (1996) Modified N-methyl-D-aspartate receptor subunit expression emerges in reeler Purkinje cells after accomplishment of the adult wild-type expression. Neurosci Res 26(4):335-43 |
| abstractText | In terms of gene expression for the N-methyl-D-aspartate (NMDA) receptor channel subunits, the adult reeler cerebellum contains two Purkinje cell populations, one expressing the zeta 1 subunit mRNA (wild-type expression) and another expressing the epsilon 1 and zeta 1 subunit mRNAs (modified expression). To clarify development of these two populations, in situ hybridization analysis was performed in the reeler cerebellum from birth to 1 year of age. The epsilon 1 subunit mRNA was not detected in any Purkinje cell clusters (PCCs) until postnatal day 21 (P21). Additional signals for the epsilon 1 subunit mRNA first appeared at P56 in a subset of PCCs, and persisted until 1 year of age. The epsilon 2 and epsilon 4 subunit mRNAs were transiently expressed in PCCs at birth, and disappeared thereafter. No signals for the epsilon 3 subunit mRNA was detected in PCCs at any postnatal stages examined. The zeta 1 subunit mRNA was expressed in all PCCs from birth through 1 year. When compared with the wild-type Purkinje cells, reeler Purkinje cells with modified expression are thus differentiated from those once accomplished the adult wild-type expression (the zeta 1 subunit alone) by down-regulation of the epsilon subunits. The present results further indicate that the onset of the modified expression is subsequent to the establishment of synaptic connectivities in the cerebellum. Copyright (C) 1996 Elsevier Science Ireland Ltd |
