| First Author | Adham IM | Year | 2008 |
| Journal | Mol Hum Reprod | Volume | 14 |
| Issue | 4 | Pages | 207-13 |
| PubMed ID | 18303090 | Mgi Jnum | J:135886 |
| Mgi Id | MGI:3794713 | Doi | 10.1093/molehr/gan009 |
| Citation | Adham IM, et al. (2008) Fas-associated factor (FAF1) is required for the early cleavage-stages of mouse embryo. Mol Hum Reprod 14(4):207-13 |
| abstractText | FAF1 was initially isolated as a Fas-associated factor and was subsequently found to interact with a subset of additional proteins that are involved in many cellular events including Fas-mediated apoptosis, heat shock signalling pathways and ubiquitin-dependent processes. Here, we describe that the 74-kDa FAF1 is ubiquitously expressed, while the expression of its post-translational-processed 49-kDa isoform is restricted to post-meiotic male germ cells. In ovary, FAF1 protein is localized predominantly in the cytoplasm of oocytes in all follicle stages. To determine the function of FAF1 in vivo, we analysed a mouse mutant line in which a gene trap vector was inserted in the Faf1 locus. The mutation disrupts the Faf1 and leads to lethality of the Faf1(GT/GT) embryos near the 2-cell stage. Analysis of FAF1 expression revealed that the protein is present in early preimplantation stages, while embryonic expression of Faf1 mRNA becomes appreciable at 4-cell stage. These results indicate that the death of Faf1(GT/GT) at the 2-cell stage may coincide with the depletion of maternal FAF1 in these embryos. Thus, our results indicate that the FAF1 gene product is necessary for early embryonic development. |
