| First Author | Braun J | Year | 2010 |
| Journal | J Cyst Fibros | Volume | 9 |
| Issue | 5 | Pages | 351-64 |
| PubMed ID | 20624691 | Mgi Jnum | J:306494 |
| Mgi Id | MGI:6709128 | Doi | 10.1016/j.jcf.2010.06.003 |
| Citation | Braun J, et al. (2010) Quantitative expression analyses of candidates for alternative anion conductance in cystic fibrosis mouse models. J Cyst Fibros 9(5):351-64 |
| abstractText | BACKGROUND: Diversity of cystic fibrosis (CF) phenotype in patients with the same CFTR-mutation raised the hypothesis that other factors modulate the phenotype including "alternative" calcium-activated anion currents (CaCC). This study compares the mRNA expression levels of candidate CaCC mediators in CF mouse models with wild type controls. METHODS: mBEST1, mBEST2, mCLC-3B, mCLC-4, mTTYH3, mTMEM16A, mTMEM16F, mTMEM16K, mCLCA1 to -6 and SLC26A9 mRNA were quantified in CF-relevant tissues in cftr(tm1Cam) and cftr(TgH(neoim)Hgu) mice and controls using real-time RT-qPCR. RESULTS: No consistent differences were observed except for mTTYH3 which was significantly down-regulated throughout the intestinal tract of cftr(tm1Cam) mice. CONCLUSIONS: Down-regulation of mTTYH3 may point towards its involvement in the complex CF pathology. However, the markedly reduced expression argues against a direct compensatory action as an alternative anion conductance. If any of the other candidates plays a role as modulator, factors other than transcriptional regulation and mRNA stability may be involved. |
