| First Author | Çuburu N | Year | 2019 |
| Journal | J Immunol | Volume | 202 |
| Issue | 4 | Pages | 1250-1264 |
| PubMed ID | 30635393 | Mgi Jnum | J:272968 |
| Mgi Id | MGI:6280811 | Doi | 10.4049/jimmunol.1800219 |
| Citation | Cuburu N, et al. (2019) A Prime-Pull-Amplify Vaccination Strategy To Maximize Induction of Circulating and Genital-Resident Intraepithelial CD8(+) Memory T Cells. J Immunol 202(4):1250-1264 |
| abstractText | Recent insight into the mechanisms of induction of tissue-resident memory (TRM) CD8(+) T cells (CD8(+) TRM) enables the development of novel vaccine strategies against sexually transmitted infections. To maximize both systemic and genital intraepithelial CD8(+) T cells against vaccine Ags, we assessed combinations of i.m. and intravaginal routes in heterologous prime-boost immunization regimens with unrelated viral vectors. Only i.m. prime followed by intravaginal boost induced concomitant strong systemic and intraepithelial genital-resident CD8(+) T cell responses. Intravaginal boost with vectors expressing vaccine Ags was far superior to intravaginal instillation of CXCR3 chemokine receptor ligands or TLR 3, 7, and 9 agonists to recruit and increase the pool of cervicovaginal CD8(+) TRM Transient Ag presentation increased trafficking of cognate and bystander circulating activated, but not naive, CD8(+) T cells into the genital tract and induced in situ proliferation and differentiation of cognate CD8(+) TRM Secondary genital CD8(+) TRM were induced in the absence of CD4(+) T cell help and shared a similar TCR repertoire with systemic CD8(+) T cells. This prime-pull-amplify approach elicited systemic and genital CD8(+) T cell responses against high-risk human papillomavirus type 16 E7 oncoprotein and conferred CD8-mediated protection to a vaccinia virus genital challenge. These results underscore the importance of the delivery route of nonreplicating vectors in prime-boost immunization to shape the tissue distribution of CD8(+) T cell responses. In this context, the importance of local Ag presentation to elicit genital CD8(+) TRM provides a rationale to develop novel vaccines against sexually transmitted infections and to treat human papillomavirus neoplasia. |
