| First Author | Sweet HO | Year | 1990 |
| Journal | Mouse Genome | Volume | 86 |
| Pages | 236-7 | Mgi Jnum | J:14266 |
| Mgi Id | MGI:62437 | Citation | Sweet HO, et al. (1990) Rough fur (ruf). Mouse Genome 86:236-7 |
| abstractText | Full text of Mouse Genome contribution: Research News: 1. Rough fur (ruf). An autosomal recessive mutation that arose in the Animal Resources colony of C3H/HeJ in 1985 has been mapped to Chromosome 9. Homozygous ruf mice have an unkempt appearance; the fur appears rough as though hairs had broken off. There is mild hyperkeratosis of the skin. Hair follicles appear to be evenly distributed and formed normally. Homozygotes of both sexes are viable and fertile. Using the MEV linkage testing stock, we found ruf is linked to dilute (d) on Chr 9 (Table 1). To determine the position of ruf in relation to other markers on Chr 9 matings were made between homozygous C3H/HeJ-ruf Apoa-lb + + Mod-1a and C57BL/6J-Apoa-la d, se Mod-lb and then then the F1 progeny were intercrossed. All the first 124 F2 progeny (+/? and ruf/ruf) were scored for ruf, d, se and then typed for the segregating isoenzymes (Table 2). We frequently search for the chromosomal locations of new mutations using intercrosses segregating for multiple isoenzyme differences and typing homozygous mutants only. Classifying all the F2 progeny for isoenzymes in this cross enabled us to test the hypothesis that typing all progeny within an intercross for isoenzymes does not give enough more information than typing the nm/nm (homozygous new mutant) only to justify the additional work of typing all the F2 mice. Information from +/? animals in an intercross is most informative for positioning loci when the order of some previous loci on the chromosome is known. The results of this cross give gene order and distances of ruf - 3.40 +/- 1.65 - Apoa-1- 14.2 +/- 3.36 - d, se - 8.45 +/- 2.59 - Mod-1. The combined estimate for ruf - d, se from all the crosses is 16.12 +/- 2.58. Table 1. 1. d +/+ ruf x d +/+ ruf; + +: 111; d +: 51; + ruf: 39; d ruf: 1; Total: 202; Recombination Estimate +/- SE: 16.24 +/- 6.81. 2. d se +/+ ruf x d se +/+ ruf; + +: 111; d +: 38; + ruf: 50; d ruf: 1; Total: 200*; Recombination Estimate +/- SE: 16.71 +/- 6.83. Totals: + +: 222; d +: 89; + ruf: 89; d ruf: 2; Total: 402; Recombination Estimate +/- SE: 16.50 +/- 4.82. 3. d ruf/+ + x d ruf/d ruf; + +: 61; d +: 13; + ruf: 10; d ruf: 60; Total: 144; Recombination Estimate +/- SE: 15.97 +/- 3.05. * These mice include the 124 mice in Table 2 that were typed for isoenzymes as well. Table 2. Genotype: nf: +; Apoa-1: ab; d, se: +; Mod-1: ab; Number of Mice: 44. Genotype: nf: +; Apoa-1: ab; d, se: +; Mod-1: a; Number of Mice: 10. Genotype: nf: +; Apoa-1: a; d, se: +; Mod-1: ab; Number of Mice: 8. Genotype: nf: +; Apoa-1: ab; d, se: +; Mod-1: b; Number of Mice: 4. Genotype: nf: +; Apoa-1: a; d, se: +; Mod-1: a; Number of Mice: 2. Genotype: nf: +; Apoa-1: b; d, se: +; Mod-1: a; Number of Mice: 1. Genotype: nf: +; Apoa-1: b; d, se: +; Mod-1: ab; Number of Mice: 1. Genotype: nf: ruf; Apoa-1: b; d, se: +; Mod-1: a; Number of Mice: 15. Genotype: nf: ruf; Apoa-1: b; d, se: +; Mod-1: ab; Number of Mice: 9. Genotype: nf: ruf; Apoa-1: ab; d, se: +; Mod-1: a; Number of Mice: 1. Genotype: nf: ruf; Apoa-1: ab; d, se: +; Mod-1: ab; Number of Mice: 1. Genotype: nf: ruf; Apoa-1: b; d, se: +; Mod-1: b; Number of Mice: 0. Genotype: nf: ruf; Apoa-1: ab; d, se: +; Mod-1: b; Number of Mice: 0. Genotype: nf: ruf; Apoa-1: a; d, se: +; Mod-1: b; Number of Mice: 0. Genotype: nf: +; Apoa-1: a; d, se: d, se; Mod-1: b; Number of Mice: 16. Genotype: nf: +; Apoa-1: ab; d, se: d, se; Mod-1: b; Number of Mice: 6. Genotype: nf: +; Apoa-1: a; d, se: d, se; Mod-1: ab; Number of Mice: 5. Genotype: nf: +; Apoa-1: ab; d, se: d, se; Mod-1: a; Number of Mice: 0. Genotype: nf: +; Apoa-1: b; d, se: d, se; Mod-1: a; Number of Mice: 0. Genotype: nf: +; Apoa-1: ab; d, se: d, se; Mod-1: ab; Number of Mice: 1. Genotype: nf: +; Apoa-1: a; d, se: d, se; Mod-1: a; Number of Mice: 0. Total: 124. The recombination estimate between each pair of loci in the intercross was calculated using E.L. GreenÕs computer program (E.L. Green, MNL 1985; 73:20) (Sweet, S-I. Oda, Taylor, L. Rowe, Davisson, S. Cook, C. Spencer, J. Sundberg). |
