| First Author | Chun HS | Year | 2003 |
| Journal | Neuroreport | Volume | 14 |
| Issue | 7 | Pages | 1055-9 |
| PubMed ID | 12802202 | Mgi Jnum | J:90062 |
| Mgi Id | MGI:3042362 | Doi | 10.1097/01.wnr.0000073426.02536.f9 |
| Citation | Chun HS, et al. (2003) Identification of genes showing differential expression in anorexia mutant mouse. Neuroreport 14(7):1055-9 |
| abstractText | Differential display (DD) PCR was applied to identify genes regulated by anorexia (anx) in mouse brain. Of the four apparently differentially expressed genes, three (H2-H4) had high homology with known genes such as apoptotic protease activating factor 1 (Apaf 1), adenylate cyclase 6 (Adcy 6), and myelin proteolipid protein (PLP). H1, designated as HIPA1, had a homology with unknown function gene fragments. Northern blot analysis confirmed HIPA1 expression induced by anorexia. A 1579 bp full-length cDNA of HIPA1 was isolated from a mouse brain cDNA library using a probe from the differentially displayed H1 fragment. Sequence analysis showed that HIPA1 had 98.7% homology to mouse hippocampus cDNA. In situ hybridization demonstrated that the HIPA1 mRNA was highly up-regulated in the hippocampus of anx/anx mouse brain. |
