| First Author | Ellmeier W | Year | 2002 |
| Journal | Immunity | Volume | 16 |
| Issue | 5 | Pages | 623-34 |
| PubMed ID | 12049715 | Mgi Jnum | J:113136 |
| Mgi Id | MGI:3664642 | Doi | 10.1016/s1074-7613(02)00309-6 |
| Citation | Ellmeier W, et al. (2002) Combined deletion of CD8 locus cis-regulatory elements affects initiation but not maintenance of CD8 expression. Immunity 16(5):623-34 |
| abstractText | Developmental stage-, subset-, and lineage-specific CD8 enhancers have been identified recently by transgenic reporter analyses. Enhancer E8(II) (CIV-4,5) is active in both immature double-positive thymocytes (DP) and mature CD8 single-positive (SP) thymocytes and T cells, whereas E8(I) (CIII-1,2) directs expression only in mature cells. In mice lacking either E8(I) (CIII-1,2) or E8(II) (CIV-4,5), there was no effect on CD8 expression in DP thymocytes. However, deletion of both enhancers resulted in variegated expression of CD8, with appearance of CD4(+)CD8(-) SP thymocytes expressing surface markers characteristic of DP thymocytes. Consequently, fewer mature CD8(+) T cells developed from the reduced pool of DP cells. These results suggest that the initiation of CD8 expression is mediated by cis-regulatory elements that are distinct from any that may be involved in maintenance of expression. |
