| First Author | Bowen S | Year | 2014 |
| Journal | J Immunol | Volume | 192 |
| Issue | 1 | Pages | 169-77 |
| PubMed ID | 24307734 | Mgi Jnum | J:207166 |
| Mgi Id | MGI:5554619 | Doi | 10.4049/jimmunol.1302398 |
| Citation | Bowen S, et al. (2014) A novel T cell subset with trans-rearranged Vgamma-Cbeta TCRs shows Vbeta expression is dispensable for lineage choice and MHC restriction. J Immunol 192(1):169-77 |
| abstractText | alphabeta T cells, which express the alpha-beta TCR heterodimer, express CD4 or CD8 coreceptors on cells that are MHC class I or MHC class II dependent. In contrast, gammadelta T cells do not express CD4 or CD8 and develop independently of MHC interaction. The factors that determine alphabeta and gammadelta lineage choice are not fully understood, and the determinants of MHC restriction of TCR specificity have been controversial. In this study we have identified a naturally occurring population of T cells expressing Vgamma-Cbeta receptor chains on the cell surface, the products of genomic trans-rearrangement between the Vgamma2 gene and a variety of Dbeta or Jbeta genes, in place of an intact TCRbeta-chain and in association with TCRalpha. Identification of this population allowed an analysis of the role of TCR variable regions in determining T cell lineage choice and MHC restriction. We found that Vgamma2(+)Cbeta(+) cells are positive for either CD4 or CD8 and are selected in an MHC class II- or MHC class I-dependent manner, respectively, thus following the differentiation pathway of alphabeta and not gammadelta cells and demonstrating that Vbeta V region sequences are not required for selection of an MHC-restricted repertoire. |
