| First Author | Biram A | Year | 2020 |
| Journal | Cell Rep | Volume | 30 |
| Issue | 6 | Pages | 1910-1922.e5 |
| PubMed ID | 32049020 | Mgi Jnum | J:287477 |
| Mgi Id | MGI:6415825 | Doi | 10.1016/j.celrep.2020.01.032 |
| Citation | Biram A, et al. (2020) B Cell Diversification Is Uncoupled from SAP-Mediated Selection Forces in Chronic Germinal Centers within Peyer's Patches. Cell Rep 30(6):1910-1922.e5 |
| abstractText | Antibodies secreted within the intestinal tract provide protection from the invasion of microbes into the host tissues. Germinal center (GC) formation in lymph nodes and spleen strictly requires SLAM-associated protein (SAP)-mediated T cell functions; however, it is not known whether this mechanism plays a similar role in mucosal-associated lymphoid tissues. Here, we find that in Peyer''''s patches (PPs), SAP-mediated T cell help is required for promoting B cell selection in GCs, but not for clonal diversification. PPs of SAP-deficient mice host chronic GCs that are absent in T cell-deficient mice. GC B cells in SAP-deficient mice express AID and Bcl6 and generate plasma cells in proportion to the GC size. Single-cell IgA sequencing analysis reveals that these mice host few diversified clones that were subjected to mild selection forces. These findings demonstrate that T cell-derived help to B cells in PPs includes SAP-dependent and SAP-independent functions. |
