| First Author | Fu Z | Year | 2017 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 58 |
| Issue | 10 | Pages | 3862-3870 |
| PubMed ID | 28763559 | Mgi Jnum | J:257569 |
| Mgi Id | MGI:6112346 | Doi | 10.1167/iovs.17-21796 |
| Citation | Fu Z, et al. (2017) Adiponectin Mediates Dietary Omega-3 Long-Chain Polyunsaturated Fatty Acid Protection Against Choroidal Neovascularization in Mice. Invest Ophthalmol Vis Sci 58(10):3862-3870 |
| abstractText | Purpose: Neovascular age-related macular degeneration (AMD) is a major cause of legal blindness in the elderly. Diets with omega3-long-chain-polyunsaturated-fatty-acid (omega3-LCPUFA) correlate with a decreased risk of AMD. Dietary omega3-LCPUFA versus omega6-LCPUFA inhibits mouse ocular neovascularization, but the underlying mechanism needs further exploration. The aim of this study was to investigate if adiponectin (APN) mediated omega3-LCPUFA suppression of neovessels in AMD. Methods: The mouse laser-induced choroidal neovascularization (CNV) model was used to mimic some of the inflammatory aspect of AMD. CNV was compared between wild-type (WT) and Apn-/- mice fed either otherwise matched diets with 2% omega3 or 2% omega6-LCPUFAs. Vldlr-/- mice were used to mimic some of the metabolic aspects of AMD. Choroid assay ex vivo and human retinal microvascular endothelial cell (HRMEC) proliferation assay in vitro was used to investigate the APN pathway in angiogenesis. Western blot for p-AMPKalpha/AMPKalpha and qPCR for Apn, Mmps, and IL-10 were used to define mechanism. Results: omega3-LCPUFA intake suppressed laser-induced CNV in WT mice; suppression was abolished with APN deficiency. omega3-LCPUFA, mediated by APN, decreased mouse Mmps expression. APN deficiency decreased AMPKalpha phosphorylation in vivo and exacerbated choroid-sprouting ex vivo. APN pathway activation inhibited HRMEC proliferation and decreased Mmps. In Vldlr-/- mice, omega3-LCPUFA increased retinal AdipoR1 and inhibited NV. omega3-LCPUFA decreased IL-10 but did not affect Mmps in Vldlr-/- retinas. Conclusions: APN in part mediated omega3-LCPUFA inhibition of neovascularization in two mouse models of AMD. Modulating the APN pathway in conjunction with a omega3-LCPUFA-enriched-diet may augment the beneficial effects of omega3-LCPUFA in AMD patients. |
