| First Author | Mian SA | Year | 2021 |
| Journal | Blood Cancer Discov | Volume | 2 |
| Issue | 2 | Pages | 135-145 |
| PubMed ID | 33778768 | Mgi Jnum | J:350599 |
| Mgi Id | MGI:6728657 | Doi | 10.1158/2643-3230.BCD-20-0161 |
| Citation | Mian SA, et al. (2021) Ectopic humanized mesenchymal niche in mice enables robust engraftment of myelodysplastic stem cells. Blood Cancer Discov 2(2):135-145 |
| abstractText | Myelodysplastic syndrome (MDS) are clonal stem cell diseases characterized mainly by ineffective hematopoiesis. Here, we present an approach that enables robust long-term engraftment of primary MDS stem cells (MDS-SCs) in mice by implantation of human mesenchymal cell-seeded scaffolds. Critically for modelling MDS, where patient sample material is limiting, mononuclear bone marrow cells containing as few as 10(4) CD34(+) cells can be engrafted and expanded by this approach with the maintenance of the genetic make-up seen in the patients. Non-invasive high-resolution ultrasound imaging shows that these scaffolds are fully perfused. Our data shows that human microenvironment but not mouse is essential to MDS-SCs homing and engraftment. Notably, the alternative niche provided by healthy donor MSCs enhanced engraftment of MDS-SCs. This study characterizes a new tool to model MDS human disease with the level of engraftment previously unattainable in mice, and offers insights into human-specific determinants of MDS-SC microenvironment. |
