| First Author | Manis JP | Year | 2002 |
| Journal | Immunity | Volume | 16 |
| Issue | 4 | Pages | 607-17 |
| PubMed ID | 11970883 | Mgi Jnum | J:113521 |
| Mgi Id | MGI:3686917 | Doi | 10.1016/s1074-7613(02)00306-0 |
| Citation | Manis JP, et al. (2002) IgH class switch recombination to IgG1 in DNA-PKcs-deficient B cells. Immunity 16(4):607-17 |
| abstractText | To assess the role of the DNA-PKcs nonhomologous DNA end-joining (NHEJ) protein in Ig heavy chain class switch recombination (CSR), we assayed CSR ability of DNA-PKcs-deficient (DP-T) B cells generated via complementation of DP-T mice with Ig heavy chain and light chain knock-in transgenes (DP-T/HC/LC mice). DP-T/HC/LC mice were severely deficient for all serum IgH isotypes except IgM and, unexpectedly, IgG1. Upon appropriate stimulation, DP-T/HC/LC B cells showed normal proliferation, germline C(H) gene transcription, and AID induction, indicating that DNA-PKcs deficiency did not affect cellular events upstream to CSR. Yet, in vitro activated DP-T/HC/LC B cells again underwent switching only to IgG1 and, like wild-type cells, frequently underwent CSR to gamma1 on both chromosomes. We conclude that DNA-PKcs is required for CSR to most C(H) genes but that CSR to gamma1 occurs via a DNA-PKcs-independent mechanism. |
