| First Author | Kim D | Year | 2016 |
| Journal | J Biol Chem | Volume | 291 |
| Issue | 34 | Pages | 17616-28 |
| PubMed ID | 27342779 | Mgi Jnum | J:236163 |
| Mgi Id | MGI:5805313 | Doi | 10.1074/jbc.M116.722736 |
| Citation | Kim D, et al. (2016) beta2-Adrenergic Receptors Chaperone Trapped Bitter Taste Receptor 14 to the Cell Surface as a Heterodimer and Exert Unidirectional Desensitization of Taste Receptor Function. J Biol Chem 291(34):17616-28 |
| abstractText | Bitter taste receptors (TAS2Rs) are G-protein-coupled receptors now recognized to be expressed on extraoral cells, including airway smooth muscle (ASM) where they evoke relaxation. TAS2Rs are difficult to express in heterologous systems, with most receptors being trapped intracellularly. We find, however, that co-expression of beta2-adrenergic receptors (beta2AR) in HEK-293T routes TAS2R14 to the cell surface by forming receptor heterodimers. Cell surface TAS2R14 expression was increased by approximately 5-fold when beta2AR was co-expressed. Heterodimer formation was shown by co-immunoprecipitation with tagged receptors, biomolecular fluorescence complementation, and merged confocal images. The dynamic nature of this interaction was shown by: a gene-dose relationship between transfected beta2AR and TAS2R14 expression, enhanced (up to 3-fold) TAS2R14 agonist stimulation of [Ca(2+)]i with beta2AR co-transfection, approximately 53% decrease in [Ca(2+)]i signaling with shRNA knockdown of beta2AR in H292 cells, and approximately 60% loss of [Ca(2+)]i responsiveness in betaAR knock-out mouse ASM. Once expressed on the surface, we detected unidirectional, conformation-dependent, interaction within the heterodimer, with beta2AR activation rapidly uncoupling TAS2R14 function ( approximately 65% desensitization). Cross-talk was independent of beta2AR internalization and cAMP/PKA, and not accompanied by TAS2R14 internalization. With prolonged beta-agonist exposure, TAS2R14 internalized, consistent with slow recycling of naked TAS2R14 in the absence of the heterodimeric milieu. In studies of ASM mechanics, rapid cross-talk was confirmed at the physiologic level, where relaxation from TAS2R14 agonist was decreased by approximately 50% with beta-agonist co-treatment. Thus the beta2AR acts as a double-edged sword: increasing TAS2R14 cell surface expression, but when activated by beta-agonist, partially offsetting the expression phenotype by direct receptor:receptor desensitization of TAS2R14 function. |
