| First Author | Peluzio MC | Year | 2003 |
| Journal | Br J Nutr | Volume | 90 |
| Issue | 1 | Pages | 3-11 |
| PubMed ID | 12844369 | Mgi Jnum | J:106130 |
| Mgi Id | MGI:3617660 | Doi | 10.1079/bjn2003870 |
| Citation | Peluzio MC, et al. (2003) Monocyte chemoattractant protein-1 involvement in the alpha-tocopherol-induced reduction of atherosclerotic lesions in apolipoprotein E knockout mice. Br J Nutr 90(1):3-11 |
| abstractText | We studied the effects of alpha-tocopheryl acetate supplementation on the development of fatty streaks and its ability to modulate the expression of monocyte chemoattractant protein (MCP)-1 in aortic lesions of apolipoprotein E knockout mice. For this purpose, 16-week-old apolipoprotein E knockout mice received alpha-tocopherol supplementation (800 mg)/kg diet) for 6 weeks. After this time, total and lipoprotein cholesterol in the serum, hepatic tocopherol, aortic lesion area and MCP-1 (protein and mRNA) expression were analysed. Our present results showed that the dietary supplementation with alpha-tocopherol did not reduce serum cholesterol nor change lipoprotein profile, but it reduced the area of the aortic lesion by 55 %. The reduction in the lesion size was correlated with the reduced expression of MCP-1 mRNA and protein, as detected by real-time quantitative polymerase chain reaction and immunohistochemistry respectively. In conclusion, the results obtained here are relevant to the study of atherosclerosis, as they correlate the effectiveness of vitamin E supplementation in inhibiting the plaque formation with diminished expression of MCP-1 at the aortic lesion. |
