| First Author | Toledo-Flores D | Year | 2019 |
| Journal | Sci Rep | Volume | 9 |
| Issue | 1 | Pages | 7286 |
| PubMed ID | 31086203 | Mgi Jnum | J:279907 |
| Mgi Id | MGI:6357556 | Doi | 10.1038/s41598-019-43765-8 |
| Citation | Toledo-Flores D, et al. (2019) Vasculogenic properties of adventitial Sca-1(+)CD45(+) progenitor cells in mice: a potential source of vasa vasorum in atherosclerosis. Sci Rep 9(1):7286 |
| abstractText | The cellular origins of vasa vasorum are ill-defined and may involve circulating or local progenitor cells. We previously discovered that murine aortic adventitia contains Sca-1(+)CD45(+) progenitors that produce macrophages. Here we investigated whether they are also vasculogenic. In aortas of C57BL/6 mice, Sca-1(+)CD45(+) cells were localised to adventitia and lacked surface expression of endothelial markers (<1% for CD31, CD144, TIE-2). In contrast, they did show expression of CD31, CD144, TIE-2 and VEGFR2 in atherosclerotic ApoE(-/-) aortas. Although Sca-1(+)CD45(+) cells from C57BL/6 aorta did not express CD31, they formed CD31(+) colonies in endothelial differentiation media and produced interconnecting vascular-like cords in Matrigel that contained both endothelial cells and a small population of macrophages, which were located at branch points. Transfer of aortic Sca-1(+)CD45(+) cells generated endothelial cells and neovessels de novo in a hindlimb model of ischaemia and resulted in a 50% increase in perfusion compared to cell-free control. Similarly, their injection into the carotid adventitia of ApoE(-/-) mice produced donor-derived adventitial and peri-adventitial microvessels after atherogenic diet, suggestive of newly formed vasa vasorum. These findings show that beyond its content of macrophage progenitors, adventitial Sca-1(+)CD45(+) cells are also vasculogenic and may be a source of vasa vasorum during atherogenesis. |
