| First Author | Janakiraman M | Year | 2022 |
| Journal | PLoS One | Volume | 17 |
| Issue | 4 | Pages | e0266589 |
| PubMed ID | 35385550 | Mgi Jnum | J:325578 |
| Mgi Id | MGI:7260921 | Doi | 10.1371/journal.pone.0266589 |
| Citation | Janakiraman M, et al. (2022) A novel CD4 knockout mouse strain with a spontaneous frameshift mutation in the CD4 locus. PLoS One 17(4):e0266589 |
| abstractText | T cells express co-receptors CD4 and CD8, which are involved in the recognition of antigen presented to T cell receptors. The expression of CD4 in thymic hematopoietic cells is crucial for the thymic development and selection of T cells. In this study, we identified a novel CD4 mutant allele that emerged spontaneously in our mouse colony. The frameshift mutation led to a truncated CD4 protein which failed to reach the plasma membrane resulting in impaired development of CD4+ helper T cells. The CRISPR mediated correction of mutant allele restored the membrane CD4 expression. Further, using an adoptive transfer of T cells, we show that this model is an ideal recipient mouse for the study of CD4+ T cells. |
