| First Author | Schad SE | Year | 2022 |
| Journal | J Exp Med | Volume | 219 |
| Issue | 6 | PubMed ID | 35604411 |
| Mgi Jnum | J:334289 | Mgi Id | MGI:7408042 |
| Doi | 10.1084/jem.20212169 | Citation | Schad SE, et al. (2022) Tumor-induced double positive T cells display distinct lineage commitment mechanisms and functions. J Exp Med 219(6) |
| abstractText | Transcription factors ThPOK and Runx3 regulate the differentiation of "helper" CD4+ and "cytotoxic" CD8+ T cell lineages respectively, inducing single positive (SP) T cells that enter the periphery with the expression of either the CD4 or CD8 co-receptor. Despite the expectation that these cell fates are mutually exclusive and that mature CD4+CD8+ double positive (DP) T cells are present in healthy individuals and augmented in the context of disease, yet their molecular features and pathophysiologic role are disputed. Here, we show DP T cells in murine and human tumors as a heterogenous population originating from SP T cells which re-express the opposite co-receptor and acquire features of the opposite cell type's phenotype and function following TCR stimulation. We identified distinct clonally expanded DP T cells in human melanoma and lung cancer by scRNA sequencing and demonstrated their tumor reactivity in cytotoxicity assays. Our findings indicate that antigen stimulation induces SP T cells to differentiate into DP T cell subsets gaining in polyfunctional characteristics. |
