| First Author | Haque M | Year | 2020 |
| Journal | iScience | Volume | 23 |
| Issue | 7 | Pages | 101333 |
| PubMed ID | 32679546 | Mgi Jnum | J:330773 |
| Mgi Id | MGI:6717500 | Doi | 10.1016/j.isci.2020.101333 |
| Citation | Haque M, et al. (2020) Stem Cell-Derived Viral Antigen-Specific T Cells Suppress HBV Replication through Production of IFN-gamma and TNF-. iScience 23(7):101333 |
| abstractText | The viral antigen (Ag)-specific CD8(+) cytotoxic T lymphocytes (CTLs) derived from pluripotent stem cells (PSCs), i.e., PSC-CTLs, have the ability to suppress hepatitis B virus (HBV) infection. After adoptive transfer, PSC-CTLs can infiltrate into the liver to suppress HBV replication. Nevertheless, the mechanisms by which the viral Ag-specific PSC-CTLs provoke the antiviral response remain to be fully elucidated. In this study, we generated the functional HBV surface Ag-specific CTLs from the induced PSC (iPSCs), i.e., iPSC-CTLs, and investigated the underlying mechanisms of the CTL-mediated antiviral replication in a murine model. We show that adoptive transfer of HBV surface Ag-specific iPSC-CTLs greatly suppressed HBV replication and prevented HBV surface Ag expression. We further demonstrate that the adoptive transfer significantly increased T cell accumulation and production of antiviral cytokines. These results indicate that stem cell-derived viral Ag-specific CTLs can robustly accumulate in the liver and suppress HBV replication through producing antiviral cytokines. |
