| First Author | Van Kaer L | Year | 2013 |
| Journal | PLoS One | Volume | 8 |
| Issue | 7 | Pages | e67821 |
| PubMed ID | 23844100 | Mgi Jnum | J:204307 |
| Mgi Id | MGI:5532234 | Doi | 10.1371/journal.pone.0067821 |
| Citation | Van Kaer L, et al. (2013) In vitro induction of regulatory CD4+CD8alpha+ T cells by TGF-beta, IL-7 and IFN-gamma. PLoS One 8(7):e67821 |
| abstractText | In vitro CD4(+) T cell differentiation systems have made important contributions to understanding the mechanisms underlying the differentiation of naive CD4(+) T cells into effector cells with distinct biological functions. Mature CD4(+) T cells expressing CD8alphaalpha homodimers are primarily found in the intestinal mucosa of men and mice, and to a lesser extent in other tissues such as peripheral blood. Although CD4(+)CD8alpha(+) T cells are easily identified, very little is known about their development and immunological functions. It has been reported, however, that CD4(+)CD8alpha(+) T cells possess regulatory properties. In this report, we present a novel in vitro differentiation system where CD4(+) T cells are stimulated to become CD4(+)CD8alpha(+) T cells in the presence of TGF-beta, IL-7 and IFN-gamma, resulting in cells with very similar features as CD4(+)CD8alpha(+) intraepithelial lymphocytes. This novel in vitro differentiation culture should provide a powerful and tractable tool for dissecting the differentiation and biological functions of CD4(+)CD8alpha(+) T cells. |
