| First Author | Chakravarti S | Year | 1991 |
| Journal | Am J Hum Genet | Volume | 49 (suppl) |
| Pages | 338 (Abstr.) | Mgi Jnum | J:12098 |
| Mgi Id | MGI:60352 | Citation | Chakravarti S, et al. (1991) Assignment of the gene for perlecan to mouse chromosome 4. Am J Hum Genet 49 (suppl):338 (Abstr.) |
| abstractText | Full text of Abstract. POSTER PRESENTATIONS - Gene Mapping: Linkage (continued). Assignment of the gene for perlecan to mouse chromosome 4. Shukti Chakravarti* and John R. Hassell. Department of Ophthalmology, University of Pittsburgh and The Eye & Ear Institute of Pittsburgh, Pittsburgh, PA. Perlecan i s a heparan sulfate proteoglycan (core protein size; Mr=400,000) present in all basement membranes. We have mapped the perlecan gene in the mouse by linkage analysis of a mouse perlecan cDNA probe (BPG-7) in recombinant inbred (RI) strains. Using this probe, 2 restriction fragment length variants (RFLVs) were evident in BamHI/EcoRI double digests of genomic DNA from progenitor inbred strains; 7.4 kb, 6.4 kb and 2.2 kb (AKR/J, DBA/2J and C3H/HeJ strains) and 10.4 kb, 5.8 kb, 2.2 kb and 1.9 kb (C57L/J and C57BL/6J strains). Twenty six C57BL/6J X DBA/2J and eighteen AKR/J X C57L/J strains were typed for these RFLVs. The strain distribution patterns of the RFLVs were compared to a mouse RI genotype database. The perlecan gene was localized on mouse chromosome 4 with close linkage to alkaline phosphatase-2 (Ak-2) and hexose-6-dehydrogenase (Gpd-1). The most likely gene order and intergene distances are: Ak-2-(1.2 +/- 0.9 cM)- perlecan- (4.3 +/- 2 cM) Gpd-1. Since the human gene has been mapped to 1, p36.1, our assignment of the mouse gene demonstrates that perlecan belongs to a common syntenic region in mouse and man. |
