| First Author | Vogel MW | Year | 1996 |
| Journal | Brain Res Dev Brain Res | Volume | 96 |
| Issue | 1-2 | Pages | 210-8 |
| PubMed ID | 8922683 | Mgi Jnum | J:36711 |
| Mgi Id | MGI:84137 | Doi | 10.1016/0165-3806(96)00122-8 |
| Citation | Vogel MW, et al. (1996) The Engrailed-2 homeobox gene and patterning of spinocerebellar mossy fiber afferents. Brain Res Dev Brain Res 96(1-2):210-8 |
| abstractText | The mouse Engrailed-2 gene, En-2, appears to be involved in cerebellar pattern formation. Homozygous null mutants for En-2 have abnormal foliation patterns in the posterior half of the cerebellum and there are changes in Purkinje and granule cell gene expression in some posterior folia, possibly reflecting changes In cell identity, We have examined the distribution of spinocerebellar messy fiber terminals in homozygous En-2(hd) null mutants to determine if En-2 is involved in regulating the pattern of afferent connectivity in the cerebellum, Spinocerebellar messy fiber terminals were labeled following WGA-HRP injections in the lumbar region of 5 homozygous En-2(hd) mutants and 4 heterozygous controls, The distribution of spinocerebellar mossy fiber terminals was consistently altered in lobules VIII and IX of the En-2(hd) mutants, The principal changes were a reduction in the number of messy fiber terminal fields in the dorsal aspect of lobule VIII and the dorsal midline field in lobule IX was fused into a single compartment, The results suggest that the deletion of En-2 expression does not transform lobule identity, al least with respect to afferent fiber positional information cues. However, the changes in foliation and afferent connectivity in the En-2 mutant support a broad role for the En-2 gene in cerebellar patterning. |
