| First Author | Weng Z | Year | 2010 |
| Journal | Blood | Volume | 116 |
| Issue | 14 | Pages | 2579-81 |
| PubMed ID | 20554973 | Mgi Jnum | J:165893 |
| Mgi Id | MGI:4838732 | Doi | 10.1182/blood-2010-03-277236 |
| Citation | Weng Z, et al. (2010) PTEN regulates collagen-induced platelet activation. Blood 116(14):2579-81 |
| abstractText | Phosphatidylinositol 3-kinase (PI3K) has been shown to play an important role in collagen-induced platelet activation, but the role(s) of PTEN, a major regulator of the PI3K/Akt signaling pathway, has not been examined in platelets. Here, we report that Pten(-/-) mouse blood contains 25% more platelets than Pten(+/+) blood and that PTEN deficiency significantly shortened the bleeding time, increased the sensitivity of platelets to collagen-induced activation and aggregation, and enhanced phosphorylation of Akt at Ser473 in response to collagen. Furthermore, we found that PP2, and the combination of apyrase, indomethacin + 1B5, respectively, inhibited collagen-induced aggregation in both PTEN(+/+) and PTEN(-/-) platelets. In contrast, LY294002 (a PI3K inhibitor) prevented the aggregation of PTEN(+/+), but not PTEN(-/-), platelets. Therefore, PTEN apparently regulates collagen-induced platelet activation through PI3K/Akt-dependent and -independent signaling pathways. |
