Primary Identifier | MGI:1857168 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Kdr |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Insertion of an in-frame lacZ gene and a loxP site flanked neomycin resistance gene cassette replaced the translated portion of the first coding exon and the proximal part of the next intron, and placed lacZ under the transcriptional control of the endogenous promoter. Neither RT-PCR using total RNA nor Western blot analysis using a carboxy terminal antibody detected gene expression in E8.5 homozygous mutant embryos. |