| First Author | Stein PL | Year | 1992 |
| Journal | Cell | Volume | 70 |
| Issue | 5 | Pages | 741-50 |
| PubMed ID | 1387588 | Mgi Jnum | J:2242 |
| Mgi Id | MGI:50766 | Doi | 10.1016/0092-8674(92)90308-y |
| Citation | Stein PL, et al. (1992) pp59fyn mutant mice display differential signaling in thymocytes and peripheral T cells. Cell 70(5):741-50 |
| abstractText | We have generated mutant mice that do not express pp59fyn, a nonreceptor protein tyrosine kinase related to pp60src, by homologous recombination in embryonic stem cells. fyn- mice did not display an overt phenotype. Because fyn is associated with the T cell receptor (TCR), thymocyte and T cell signaling was analyzed in the mutant background. Cross-linking of TCR-CD3 in thymocytes led to markedly reduced calcium fluxes and abrogated proliferation, whereas mature splenic T cells retained largely normal proliferation despite depressed calcium movements and IL-2 production. Similarly, proliferation induced by Thy-1 cross-linking was reduced in thymocytes but not in splenic T cells. fyn- thymocytes were impaired at a late stage of maturation and showed limited clonal deletion to the Mls-1a self-super-antigen but not to staphylococcal enterotoxin A. These results implicate fyn as a critical component in TCR signaling in thymocytes and, potentially, in the process that determines T cell repertoire in the adult mouse. |
