| First Author | Houri-Haddad Y | Year | 2002 |
| Journal | J Dent Res | Volume | 81 |
| Issue | 6 | Pages | 395-8 |
| PubMed ID | 12097431 | Mgi Jnum | J:105950 |
| Mgi Id | MGI:3617059 | Doi | 10.1177/154405910208100608 |
| Citation | Houri-Haddad Y, et al. (2002) Interferon-gamma deficiency attenuates local P. gingivalis-induced inflammation. J Dent Res 81(6):395-8 |
| abstractText | Infection with the periodontal pathogen Porphyromonas gingivalis causes a strong local inflammatory reaction. Using IFNgamma-deficient mice, we tested the hypothesis that the absence of IFNgamma would result in a reduction of the local pro-inflammatory response to P. gingivalis. Cytokine secretion by macrophages from IFNgamma(-/-) animals was significantly attenuated. Addition of IFNgamma restored cytokine secretion. In vivo injection of P. gingivalis into subcutaneous chambers increased the intra-chamber leukocyte counts and TNFalpha and IL-1beta levels. This increase was significantly lower in the IFNgamma(-/-) mice. Local reconstitution of IFNgamma(-/-) mice at the site of inflammation with the IFNgamma gene increased the levels of TNFalpha and decreased the IL-10 levels. Anti-P. gingivalis IgG1 levels, a marker of Th2 response, were higher in immunized IFNgamma(-/-) than in IFNgamma(+/+) mice. The results suggest that lack of IFNgamma reduced the amplitude of the local pro-inflammatory response without decreasing the humoral protective response. The higher IgG1/IgG2a ratio observed supports the possibility of a Th2-dominant response in IFNgamma-deficient animals. |
