| First Author | Goes N | Year | 1995 |
| Journal | Transplantation | Volume | 60 |
| Issue | 12 | Pages | 1560-4 |
| PubMed ID | 8545890 | Mgi Jnum | J:31381 |
| Mgi Id | MGI:78882 | Doi | 10.1097/00007890-199560120-00031 |
| Citation | Goes N, et al. (1995) Acute renal injury in the interferon-gamma gene knockout mouse: effect on cytokine gene expression. Transplantation 60(12):1560-4 |
| abstractText | We studied major histocompatibility complex (MHC) and cytokine mRNA induction after renal injury in the absence of interferon-gamma (IFN-gamma) using IFN-gamma gene knockout (GKO) mice. The left renal pedicle of normal (wild-type) and GKO BALB/c mice was clamped for 60 minutes; cytokine and MHC mRNA expression were monitored in the injured kidney and compared to the contralateral control kidney. After a single episode of ischemic injury, the expression of mRNA for MHC class I and II, interleukin-2, interleukin-10, granulocyte-macrophage colony-stimulating factor, tumor necrosis factor-alpha, and transforming growth factor-beta 1 was increased in wild-type and GKO mice, whereas preproepidermal growth factor (ppEGF) was reduced. IFN-gamma expression was induced in wild-type mice but absent in the GKO mice. Therefore, local injury was equally effective in both wild-type and GKO mice with equivalent cytokine and MHC mRNA induction, proving that local tissue injury can induce MHC expression by non-IFN-gamma factors. |
