| First Author | Andrews C | Year | 2023 |
| Journal | Front Immunol | Volume | 14 |
| Pages | 1021824 | PubMed ID | 37153622 |
| Mgi Jnum | J:336330 | Mgi Id | MGI:7481540 |
| Doi | 10.3389/fimmu.2023.1021824 | Citation | Andrews C, et al. (2023) IL-27 induces an IFN-like signature in murine macrophages which in turn modulate colonic epithelium. Front Immunol 14:1021824 |
| abstractText | Mucosal delivery of IL-27 has been shown to have a therapeutic benefit in murine models of inflammatory bowel disease (IBD). The IL-27 effect was associated with phosphorylated STAT1 (pSTAT1), a product of IL27 receptor signaling, in bowel tissue. To determine whether IL-27 acted directly on colonic epithelium, murine colonoids and primary intact colonic crypts were shown to be unresponsive to IL-27 in vitro and to lack detectable IL-27 receptors. On the other hand, macrophages, which are present in inflamed colon tissue, were responsive to IL-27 in vitro. IL-27 induced pSTAT1 in macrophages, the transcriptome indicated an IFN-like signature, and supernatants induced pSTAT1 in colonoids. IL-27 induced anti-viral activity in macrophages and MHC Class II induction. We conclude that the effects of mucosal delivery of IL-27 in murine IBD are in part based on the known effects of IL27 inducing immunosuppression of T cells mediated by IL-10. We also conclude that IL-27 has potent effects on macrophages in inflamed colon tissue, generating mediators that in turn act on colonic epithelium. |
