| First Author | Pelanda R | Year | 1996 |
| Journal | Immunity | Volume | 5 |
| Issue | 3 | Pages | 229-39 |
| PubMed ID | 8808678 | Mgi Jnum | J:35357 |
| Mgi Id | MGI:82806 | Doi | 10.1016/s1074-7613(00)80318-0 |
| Citation | Pelanda R, et al. (1996) A prematurely expressed Ig kappa transgene, but not a V kappa J kappa gene segment targeted into the Ig kappa locus, can rescue B cell development in lambda 5-deficient mice. Immunity 5(3):229-239 |
| abstractText | We generated surrogate light chain (SLC)-deficient mice carrying either a V kappa J kappa-C kappa transgene under the control of the kappa promoter and intron enhancer or a V kappa J kappa gene segment targeted into its physiological position. Efficient rescue of B cell development was seen in the former and partial rescue in the latter. This difference corresponded to a developmentally earlier onset of kappa chain expression from the conventional than from the targeted transgene. Thus, a kappa chain can substitute for SLC in development. However, mechanisms controlling gene expression in addition to gene rearrangements appear to restrict kappa chain expression largely to a cellular compartment into which mu chain-expressing B cell progenitors are selected with the help of the SLC. |
