| First Author | Löffert D | Year | 1996 |
| Journal | Immunity | Volume | 4 |
| Issue | 2 | Pages | 133-44 |
| PubMed ID | 8624804 | Mgi Jnum | J:31600 |
| Mgi Id | MGI:79086 | Doi | 10.1016/s1074-7613(00)80678-0 |
| Citation | Loffert D, et al. (1996) Surrogate light chain expression is required to establish immunoglobulin heavy chain allelic exclusion during early B cell development. Immunity 4(2):133-44 |
| abstractText | Allelic exclusion at the IgH locus was examined in B lineage cells of wild-type mice and mice unable to express the surrogate light chain molecule lambda 5 using a single-cell PCR approach. By analyzing B precursor cells containing two VHDHJH rearrangements, we found that in wild-type animals, cells are allelically excluded as soon as mu chains are expressed. Furthermore, we provide evidence that in cells expressing D mu proteins VH-->DHJH rearrangement is inhibited. In contrast, in the absence of lambda 5 protein, B precursor cells were allelically included, indicating that allelic exclusion at the IgH locus requires expression of the pre-B cell receptor either containing a mu chain or a D mu chain. However, although mu chain double-producing B precursor cells are generated in lambda 5-deficient mice, such cells were not detected among surface immunoglobulin positive B cells. |
