| First Author | Hu Y | Year | 2022 |
| Journal | Mol Immunol | Volume | 143 |
| Pages | 105-113 | PubMed ID | 35114487 |
| Mgi Jnum | J:336636 | Mgi Id | MGI:6887286 |
| Doi | 10.1016/j.molimm.2022.01.010 | Citation | Hu Y, et al. (2022) IL-4 plays an essential role in DnaJ-DeltaA146Ply-mediated immunoprotection against Streptococcus pneumoniae in mice. Mol Immunol 143:105-113 |
| abstractText | The fusion protein DnaJ-DeltaA146Ply is protective against pneumococcal infections in mice. However, we found that immunized IL-4(-/-) mice showed significant lower survival rates and higher bacterial loads than did wild-type (WT) mice after being challenged. We explored the role of IL-4 in the protective immunity conferred by DnaJ-DeltaA146Ply. Our results showed that there were no significant differences in antibody titers between immunized WT mice and IL-4(-/-) mice. The bacterial loads of passively immunized IL-4(-/-) mice were significantly higher than those of WT mice, while mice immunized with anti-DnaJ-DeltaA146Ply serum from WT and IL-4(-/-) mice showed similar capacity for bacterial clearance. DnaJ-DeltaA146Ply-dependent phagocytosis of IL-4(-/-) neutrophils was significant decreased compared with that of WT neutrophils. The levels of Syk and phosphor-Syk in IL-4(-/-) neutrophils were decreased compared with those in WT neutrophils. Additionally, Splenocytes in IL-4(-/-) mice triggered significantly higher levels of IFN-gamma and IL-17A than did splenocytes in WT mice. Taken together, our findings illustrate that IL-4 deficiency does not influence the antibody production or antibody effect, but change the cellular immune response induced by DnaJ-DeltaA146Ply. Additionally, IL-4 can enhance the antibody-dependent phagocytosis of neutrophils partially by activating Syk and participate in the protective immunity induced by DnaJ-DeltaA146Ply. |
