| First Author | Morimoto M | Year | 2006 |
| Journal | J Immunol | Volume | 176 |
| Issue | 1 | Pages | 491-5 |
| PubMed ID | 16365442 | Mgi Jnum | J:126259 |
| Mgi Id | MGI:3760922 | Doi | 10.4049/jimmunol.176.1.491 |
| Citation | Morimoto M, et al. (2006) Functional importance of regional differences in localized gene expression of receptors for IL-13 in murine gut. J Immunol 176(1):491-5 |
| abstractText | IL-13 induces a STAT6-dependent hypercontractility of intestinal smooth muscle that is mediated by binding to the IL-13Ralpha1 component of the type 2 IL-4R that is linked to STAT6. IL-13 also binds to the IL-13Ralpha2 that is not linked to STAT6 and functions to limit the effects of IL-13 in vivo. In this study we assessed the contributions of regional and cellular differences in the distribution of the IL-13R components to the physiological regulation of smooth muscle function in wild-type mice and mice deficient in STAT6 or IL-13Ralpha2. The expression of IL-13 and IL-13Ralpha2 was higher in colon than in small intestine. Laser capture microdissection of specific cell types revealed that the expression of IL-13Ralpha2 was higher in the smooth muscle layer compared with levels in the epithelial cells of the mucosa. In contrast, there was a uniform distribution of IL-13alpha1 in smooth muscle, epithelia, and myenteric neurons. The significant hypercontractility of smooth muscle in mice deficient in IL-13Ralpha2, but not in STAT6, shows the physiological importance of IL-13 binding to IL-13Ralpha2. The pronounced differences in the expression of IL-13Ralpha2 suggest that the gut has developed sophisticated mechanisms for controlling the physiological and pathophysiological activities of IL-13. |
