| First Author | Noben-Trauth N | Year | 1996 |
| Journal | Transgenic Res | Volume | 5 |
| Issue | 6 | Pages | 487-91 |
| PubMed ID | 8840532 | Mgi Jnum | J:36386 |
| Mgi Id | MGI:83819 | Doi | 10.1007/BF01980214 |
| Citation | Noben-Trauth N, et al. (1996) Efficient targeting of the IL-4 gene in a BALB/c embryonic stem cell line. Transgenic Res 5(6):487-91 |
| abstractText | Embryonic stem (ES) cell lines have been derived from the inner cell mass of day 3.5 blastocysts of the inbred mouse strain BALB/cJ. Twenty-three lines were karyotyped and three were selected for injection into C57BL/6J host blastocysts. Two of the three lines, BALB/c-I and BALB/c-IV, produced germ-line chimaeras. The suitability of the BALB/c-I line for gene targeting experiments was tested by transfecting a targeting construct for the interleukin-4 (IL-4) gene. Transfected BALB/c-I cells exhibited efficient homologous recombination of the targeting vector and transmitted the induced mutation through the germline. This newly-characterized BALB/c-ES cell line thus provides an alternative to the traditional 129-derived and the recently described C57BL/6 embryonic stem cell lines, and will be useful in disrupting genes involved in the immune system. Furthermore, the genetically pure BALB/c IL-4 deficient mice will aid in studying the role of IL-4 in several infectious disease models in which the BALB/c mouse is a susceptible strain. |
