| First Author | Chida Y | Year | 2004 |
| Journal | Neuroimmunomodulation | Volume | 11 |
| Issue | 6 | Pages | 419-24 |
| PubMed ID | 15467358 | Mgi Jnum | J:105444 |
| Mgi Id | MGI:3615083 | Doi | 10.1159/000080153 |
| Citation | Chida Y, et al. (2004) Electric foot-shock stress drives TNF-alpha production in the liver of IL-6-deficient mice. Neuroimmunomodulation 11(6):419-24 |
| abstractText | OBJECTIVES: Accumulating evidence has shown that interleukin-6 (IL-6) has pleiotropic effects on a variety of biological functions, including its antiapoptotic potential during liver injury. Our previous work demonstrated that restraint stress-induced elevation of plasma IL-6 negatively regulates plasma tumor necrosis factor-alpha (TNF-alpha). Herein, we further clarified the mechanism underlying the above finding and investigated the effect of IL-6 on liver apoptosis triggered by stress. METHODS: Male C57BL/6J and IL-6-deficient C57BL/SV129 mice were exposed to 1 h of electric foot-shock stress. Thereafter, the serum, liver and spleen TNF-alpha levels were measured at several time points. Serum alanine aminotransferase (ALT), liver caspase-3 and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) activities were analyzed to evaluate the severity of liver injury and apoptosis. RESULTS: The liver, but not the spleen, of the IL-6-deficient mice exhibited a significant increase in TNF-alpha level after stress in parallel with serum TNF-alpha elevation, whereas no such TNF-alpha responses were found in the wild animals. No significant differences in stress-induced elevation of serum ALT levels, liver caspase-3 activities and the number of TUNEL-positive hepatocytes were found between the wild and IL-6-deficient mice. CONCLUSIONS: Taken together, these results indicate that IL-6 may play a critical role in suppressing TNF-alpha production in the liver, thereby decreasing the blood TNF-alpha level. In contrast, IL-6 secretion was shown to have no protective effect on stress-triggered liver injury. |
